DNA Methylation Test Plus

About this test

Key Benefits

• Gain insight into how genetic variation may influence methylation and cellular processes

• Simple At-Home Testing: Collect your sample easily using a saliva test

• Comprehensive Methylation Insights: Covers detoxification, gene regulation, and nutrient pathways

• Personalized Insights: Understand how your body may respond to methylation-related factors

• Supports Informed Decisions: Helps guide lifestyle, nutrition, and supplementation choices

What This Test Measures

This test analyzes 23 genetic markers involved in methylation pathways related to folate metabolism, homocysteine balance, detoxification, vitamin transport, and cellular function.

Folate and B Vitamin Pathways

These genes are involved in how the body uses folate and vitamin B12, which support methylation, DNA production, nervous system function, and cellular health.

Methylation and Homocysteine Balance

These genes are linked to methyl donor activity and homocysteine regulation, processes connected to neurotransmitter activity, cardiovascular health, and normal cellular function.

Detoxification and Cellular Protection

These genes support pathways related to sulfur metabolism, oxidative stress, antioxidant defense, and detoxification.

Vitamin Transport and Gene Regulation

These genes are involved in B vitamin transport, DNA methylation, and epigenetic regulation, providing insight into methylation-related cellular processes.

About Methylation and Health

Methylation is a biochemical process involved in gene regulation, DNA repair, neurotransmitter balance, detoxification, and homocysteine metabolism.

It depends on connected pathways that use nutrients such as folate, vitamin B12, vitamin B6, choline, betaine, and other methyl donors. DNA Methylation Test Plus looks at a broader set of genes than the standard methylation test, giving additional insight into vitamin transport, detoxification, oxidative stress, and epigenetic regulation.

Genetic variation in these pathways may influence how efficiently they function, providing useful context for energy, mood, cognitive function, detoxification capacity, and overall cellular health.

How It Works

1. Order Your Kit
   Purchase your test online

2. Collect Your Sample
   Provide a saliva sample at home using the collection kit

3. Send It Back
   Mail your sample to the laboratory using the prepaid return envelope

4. Receive Your Results
   Access your detailed digital results within 4–8 weeks

About the Result Reports

Once your analysis is complete, you will receive three separate DNA reports based on your selected tests. These are typically delivered at the same time.

Each report is designed to be clear and easy to understand, including your genetic results along with explanations and personalized insights.

You will also have access to an example report, allowing you to preview the structure and content in advance.

If you need help interpreting your results, our support team is available to assist you.

Please note: Reports are available in English only.

Sample Collection

The sample is collected using a simple saliva test at home. You provide a small saliva sample in the collection tube, which is then sent to the laboratory for analysis.

This method is non-invasive, convenient, and easy to perform, making it suitable for home testing.

ISO-Certified Lab and Analysis

Your sample is analyzed in an ISO-certified laboratory using the Illumina GSA Microarray, a type of SNP genotyping.

This technology enables accurate and reliable analysis of genetic markers associated with methylation, gene regulation, and metabolic pathways.

Biomarkers included

• BHMT: BHMT (Betaine-Homocysteine S-Methyltransferase): BHMT is an enzyme that reflects the body’s ability to metabolize homocysteine, a process important for cardiovascular and neurological health. BHMT catalyzes the conversion of homocysteine to methionine using betaine as a methyl donor. Dysregulation of BHMT can lead to elevated homocysteine levels, which are associated with cardiovascular disease an
• PDXK: The PDXK gene encodes the enzyme pyridoxal kinase, which is essential for converting vitamin B6 into its active form, pyridoxal-5′-phosphate (PLP). PLP functions as a coenzyme in over 100 enzymatic reactions, many of which are involved in amino acid metabolism, neurotransmitter synthesis, and energy production. Variants in the PDXK gene may impair this conversion, potentially leading to functional
• MTR: MTR (5-Methyltetrahydrofolate-Homocysteine Methyltransferase): MTR is a critical enzyme involved in the remethylation pathway of homocysteine metabolism. It catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate (5-MTHF) to homocysteine, producing methionine and tetrahydrofolate (THF). Methionine is a precursor for S-adenosylmethionine (SAM), a universal methyl donor essential for
• GNMT: GNMT (Glycine N-Methyltransferase) is an important enzyme involved in methionine metabolism and liver detoxification. It helps regulate homocysteine levels by converting excess methionine into sarcosine, preventing harmful methionine accumulation and supporting liver health. Dysfunction of GNMT is associated with liver conditions such as fatty liver disease and liver cancer. Ongoing research highl
• TYMS: The TYMS gene encodes thymidylate synthase, a key enzyme involved in the synthesis of thymidine, one of the four nucleotides required for DNA replication and repair. This enzyme relies on active folate (5,10-methylene-THF) to function efficiently, linking TYMS closely to folate metabolism. Variants in the TYMS gene can affect folate availability at the cellular level and may alter how the body res
• TCN2: The TCN2 gene encodes transcobalamin II, a protein responsible for transporting vitamin B12 (cobalamin) from the bloodstream into cells. Once vitamin B12 is absorbed in the gut, it must bind to transcobalamin to be delivered to tissues where it is used for DNA synthesis, red blood cell formation, and neurological function. Genetic variations in TCN2 can reduce the efficiency of B12 transport, pote
• PEMT: PEMT (Phosphatidylethanolamine N-Methyltransferase): PEMT is an enzyme that converts phosphatidylethanolamine to phosphatidylcholine in the liver, a key step for maintaining cell membrane structure and enabling VLDL secretion. Its activity affects liver function, fat metabolism, and choline needs, with dysregulation linked to liver and cardiovascular disease risk.
• DHFR: DHFR (Dihydrofolate Reductase) is an enzyme that plays a key role in DNA synthesis and repair. It catalyzes the conversion of dihydrofolate to tetrahydrofolate, a necessary cofactor for producing purines, thymidylate, and certain amino acids. This process is essential for cell growth and division, making DHFR a critical target in cancer treatment, where inhibitors like methotrexate block its activ
• FOLH1: FOLH1 (Folate Hydrolase 1), also known as Prostate-Specific Membrane Antigen (PSMA), is a gene involved in folate metabolism and the activation of folate for DNA synthesis and repair. It plays a key role in cell growth and division. FOLH1 is notably expressed in prostate cancer cells, making it a valuable target for cancer diagnosis and therapy.
• DNMT3B: DNMT3B (DNA Methyltransferase 3 Beta) is a gene that encodes an enzyme involved in DNA methylation — an important epigenetic mechanism that regulates gene expression. DNMT3B plays a critical role in development and has been linked to conditions such as ICF syndrome and various types of cancer.
• CUBN: CUBN (Cubilin) is a multifunctional receptor protein involved in the absorption and transport of key nutrients, including vitamin B12 and various proteins such as albumin. It plays a vital role in the kidneys and intestines, where it helps reabsorb filtered proteins and supports nutrient uptake. In the kidney, CUBN works alongside megalin to maintain protein balance, while in the gut, it is essent
• CHDH: CHDH (Choline Dehydrogenase) is an important enzyme involved in choline metabolism, converting choline into betaine. This conversion supports the production of acetylcholine, a key neurotransmitter, and helps regulate homocysteine levels, which is important for cardiovascular health. CHDH activity influences choline availability, affecting liver function, brain development, and nervous system heal
• MTHFS: The MTHFS gene encodes methenyltetrahydrofolate synthetase, an enzyme involved in folate metabolism. It plays a crucial role in maintaining the pool of active folate derivatives used for methylation, DNA synthesis, and neurotransmitter production. MTHFS regulates the conversion of various folate forms and helps maintain the balance of one-carbon units essential for cellular function. Variants in t
• MTHFR: MTHFR (Methylenetetrahydrofolate Reductase): MTHFR is an enzyme that plays a central role in folate metabolism and the regulation of homocysteine levels. It converts 5,10-methylenetetrahydrofolate into 5-methyltetrahydrofolate, enabling the remethylation of homocysteine to methionine — a precursor to S-adenosylmethionine (SAM), the body’s primary methyl donor. Variants in the MTHFR gene, such as C
• MAT1A: MAT1A (Methionine Adenosyltransferase I, Alpha) is an enzyme that produces S-adenosylmethionine (SAMe), the body’s primary methyl donor for key metabolic processes. It plays a central role in methionine metabolism, influencing gene regulation, cell growth, and detoxification. Reduced MAT1A activity is linked to liver disorders such as cirrhosis and hepatocellular carcinoma.
• FUT2: FUT2 (Fucosyltransferase 2) is a gene that encodes an enzyme involved in the synthesis of histo-blood group antigens and the determination of secretor status. It catalyzes the transfer of fucose to specific molecules, enabling the production of glycoproteins and glycolipids found in bodily fluids and epithelial tissues. FUT2 activity influences the expression of the H antigen — a key precursor to
• COMT: COMT (Catechol-O-Methyltransferase): COMT is an enzyme that plays a key role in the metabolism of catecholamines, including dopamine, epinephrine, and norepinephrine. It is important for regulating neurotransmitter levels in the brain and is linked to psychiatric disorders such as schizophrenia. COMT also contributes to the body’s pain response and has been studied in relation to pain sensitivity
• AHCY: AHCY: Encodes the enzyme adenosylhomocysteinase, which plays a key role in converting S-adenosylhomocysteine to homocysteine, an essential step in the body's methylation processes and detoxification.
• SHMT1: SHMT1 (Serine Hydroxymethyltransferase 1): SHMT1 is an enzyme that catalyzes the conversion of serine and tetrahydrofolate into glycine and methylenetetrahydrofolate. This process is essential for nucleotide synthesis and methylation reactions, which support DNA replication and repair. SHMT1 plays a key role in cell growth and genetic stability, and its activity in folate metabolism is linked to c
• MTHFD1L: The MTHFD1L gene encodes a mitochondrial enzyme involved in the folate cycle and one-carbon metabolism, specifically in the conversion of formate to 10-formyl-THF. This process supports purine synthesis and methylation reactions, which are essential for DNA and RNA production and cellular repair. Genetic variations in MTHFD1L can affect mitochondrial folate metabolism and have been associated with
• MTRR: MTRR (Methionine Synthase Reductase): MTRR is an enzyme that plays a critical role in regenerating methylcobalamin, supporting the continuous function of MTR in homocysteine metabolism. It is essential for maintaining proper methionine levels and normal DNA synthesis. Mutations in MTRR are linked to homocystinuria, which can contribute to developmental and neurological complications. Understanding
• CBS: CBS (Cystathionine beta-synthase): CBS is an enzyme essential for sulfur metabolism, playing a key role in converting homocysteine to cysteine. This process supports the production of glutathione, an important antioxidant for protecting cells against oxidative stress. CBS also participates in the transsulfuration pathway, which regulates sulfur-containing amino acids and hydrogen sulfide, a signal
• MTHFD1: MTHFD1 (Methylenetetrahydrofolate Dehydrogenase 1): MTHFD1 is a key enzyme in the folate metabolism pathway, catalyzing the conversion of tetrahydrofolate (THF) derivatives into forms used for the synthesis of DNA, RNA, and amino acids. It plays a vital role in one-carbon metabolism, influencing cellular methylation reactions and nucleotide production. Mutations or dysregulation of MTHFD1 can disr

How to prepare

No special preparation is required before taking the test. Avoid eating, drinking, smoking, or chewing gum for at least 30 minutes before sample collection to ensure sample quality.

Frequently asked questions

Why are methylation pathways important?

Methylation pathways are involved in processes related to DNA repair, neurotransmitter balance, detoxification, and homocysteine regulation. These pathways also help the body utilize important nutrients such as folate, vitamin B12, and vitamin B6.

What nutrients are connected to methylation?

Methylation depends on several nutrients and cofactors, including folate, vitamin B12, vitamin B6, choline, and betaine. Genetic variation may influence how efficiently the body utilizes these nutrients within methylation pathways.

How can I use the results in my daily life?

The results provide insight into how your body may manage methylation and related biochemical pathways, supporting more informed decisions around nutrition, supplementation, and lifestyle choices.

What makes this test different from a general metabolism test?

This test focuses specifically on methylation pathways, including folate metabolism, homocysteine regulation, and methyl donor activity. It is designed to provide insight into biochemical processes related to gene regulation, detoxification, and cellular function, rather than broader metabolic traits such as weight or blood sugar regulation.

Can I receive the raw data from my test?

No. We provide your results in a comprehensive report with the relevant values and interpretations. We do not provide the raw laboratory data.

Customer reviews

4.5/5 (2)

• 4/5 — Took a bit longer than expected to arrive, but the methylation breakdown is very detailed.
• 5/5 — Very easy saliva collection and the report gave me exactly the data I needed for my supplements.