Melatonin Test

About this test

Key Benefits

• Sleep Rhythm Insight: Measures melatonin, a hormone involved in sleep timing and circadian rhythm.

• Relevant for Sleep Problems: May be useful when falling asleep, staying in rhythm or waking rested feels difficult.

• At Home Saliva Collection: Collect your saliva sample at home using the included test kit.

• Digital Results in 10 to 15 Business Days: Access your laboratory results online.

Why Melatonin Balance Matters

Melatonin is closely linked to how the body prepares for sleep, maintains a stable sleep wake rhythm and responds to darkness and light.

When this rhythm is disrupted, it may affect how easily you fall asleep, how rested you feel in the morning and how stable your energy feels during the day.

Sleep related problems can also influence focus, mood, recovery, motivation and overall wellbeing. Measuring melatonin can help provide a clearer picture of whether your circadian rhythm may be part of the reason sleep feels inconsistent or unrefreshing.

How It Works

1. Order Your Kit
Purchase your test online.

2. Collect Your Sample
Collect a saliva sample at home using the included collection tube.

3. Send It Back
Mail your sample to the laboratory using the included return materials.

4. Receive Your Results
Access your digital laboratory results online in 10 to 15 business days.

Saliva Sample Collection

The sample is collected using a saliva sample at home. Collect your sample according to the included instructions and send it to the laboratory for analysis.

Because melatonin is linked to circadian rhythm, correct sample timing and preparation are important for reliable interpretation.

ISO Certified Lab and Analysis

The sample is analysed at an ISO certified laboratory using the ELISA, Enzyme Linked Immunosorbent Assay, method.

Biomarkers included

• Melatonin: Melatonin is a hormone primarily produced by the pineal gland in the brain. It plays a key role in regulating the body’s internal clock, including the sleep-wake cycle. Melatonin levels naturally rise in the evening, peak overnight, and fall by morning, helping signal the body to prepare for rest. It also supports other functions such as immune response and antioxidant protection.

How to prepare

The sample should be collected in the **morning on an empty stomach**. Send your sample for analysis on **Monday or Tuesday.** If your gums are bleeding, avoid collecting the sample until this has resolved. **7 days before testing** Avoid hormone intake or melatonin supplements unless you want this reflected in your result. **24 hours before testing** Avoid alcohol, caffeine, nicotine and intense exercise. **30 minutes before testing** Do not brush your teeth, drink, eat, smoke or chew gum. Follow the included instructions carefully before collecting your sample.

Frequently asked questions

Do I need to avoid melatonin supplements before testing?

Yes. Avoid melatonin or hormone intake for 7 days before testing, unless you want this reflected in your result.

Why must I send my sample on a Monday?

The sample should be collected and mailed on a Monday. This ensures the postage and laboratory processing occur within the same week, maintaining the stability and integrity of the saliva sample before the weekend.

How is this test different from a Cortisol test?

Melatonin is the primary sleep regulator, while cortisol is the stress hormone that helps you wake up. These two hormones work in an inverse relationship; checking both provides a complete picture of your 24-hour sleep-wake cycle and can identify if stress is interfering with your ability to rest.

Customer reviews

4.8/5 (5)

• 5/5 — Instructions were very clear and I got my results right around the 10-day mark.
• 5/5 — Fast data, simple collection.
• 5/5 — Easy to use!
• 4/5 — Good kit, just make sure you remember to mail it on a Monday like the instructions say.
• 5/5 — The report was easy to read and confirmed why my sleep has been so off lately.